RealHelix™ qRT-PCR Kit [Probe] 의 bulk version 입니다.

대량구매를 원하시는 경우 sale@nanohelix.net 으로 문의주시기 바랍니다.

RealHelix™ qRT-PCR Kit [Probe]는 probe를 사용하여 다양한 표적 증폭 및 검출을 위한 real-time RT-PCR에 최적화된 제품입니다. Reverse transcriptase, chemically-modified hot-start Taq polymerase (Hot-Taq polymerase) 및 RNase inhibitor가 최적화된 조건으로 혼합되어 있어서 높은 특이도(specificity)와 민감도(sensitivity)를 가지고 있습니다. Buffer mix는 반응에 최적화된 buffer components와 함께 Mg²⁺, dNTPs 및 stabilizing agent가 혼합되어 있습니다. Bulk version은 enzyme mix와 buffer mix만 제공되며, ROX reference dye는 요청 시 별도 제공됩니다.

Figure 1. Multiplex (four targets) qRT-PCR with RealHelix™ qRT-PCR Kit [Probe].

Total RNAs from human whole blood were analyzed using four different sets of target-specific primers and probes. The probes were labeled with FAM, HEX, Texas Red, or Cy5. The multiplex reaction was performed using the RealHelix™ qRT-PCR Kit [Probe] on a real-time PCR instrument, the CFX96 (Bio-Rad, USA).

Figure 2. Comparison of RealHelix™ qRT-PCR Kit [Probe] and qRT-PCR Kit [Probe type] (the previous version).

10-fold serial dilutions (10, 1, 0.1, and 0.01 ng) of the human total RNA from whole blood were analyzed using a human globin gene specific primer set. RealHelix™ qRT-PCR Kit [Probe] shows earlier Ct and more reliable amplifications than the previous version. Reactions were run on a real-time PCR instrument, CFX96 (Bio-Rad, USA).

Storage

• Store below -20 ℃
• ROX dye should be stored in a dark place

Shelf life

• 12 months